车叶草苷

反应条件: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

实验步骤: Asperuloside (1.0 g) was dissolved in distilled water (10 ml) and adjusted to pH=10 by adding a small amount of a saturated solution of BaO in methanol. This solution was allowed to stand for a few minutes to become cloudy white, which, after neutralization and concentration, was treated by silica gel column chromatography (Waco Gel C-300, 50 g, solvent: chloroform-methanol system). Silica gel column chromatography gave deacetyl asperulosidic acid methyl ester having Rf value=0.79 (chloroform-methanol-water=6:6:1, visualized with 50(%)-sulfuric acid) on TLC, as white needles (1.80 mg). to stand for a few minutes which, after neutralization and concentration was treated by silica gel column chromatography (Waco Gel C-300, 50 g, solvent: chloroform-methanol system)

反应条件: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

实验步骤: Asperuloside (1.0 g) was dissolved in distilled water (10 ml) and adjusted to pH=10 by adding a small amount of a saturated solution of BaO in methanol. This solution was allowed to stand for a few minutes to become cloudy white, which, after neutralization and concentration, was treated by silica gel column chromatography (Waco Gel C-300, 50 g, solvent: chloroform-methanol system). Silica gel column chromatography gave deacetyl asperulosidic acid methyl ester having Rf value=0.79 (chloroform-methanol-water=6:6:1, visualized with 50(%)-sulfuric acid) on TLC, as white needles (1.80 mg). to stand for a few minutes which, after neutralization and concentration was treated by silica gel column chromatography (Waco Gel C-300, 50 g, solvent: chloroform-methanol system)

反应条件: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

实验步骤: Asperuloside (1.0 g) was dissolved in distilled water (10 ml) and adjusted to pH=10 by adding a small amount of a saturated solution of BaO in methanol. This solution was allowed to stand for a few minutes to become cloudy white, which, after neutralization and concentration, was treated by silica gel column chromatography (Waco Gel C-300, 50 g, solvent: chloroform-methanol system). Silica gel column chromatography gave deacetyl asperulosidic acid methyl ester having Rf value=0.79 (chloroform-methanol-water=6:6:1, visualized with 50(%)-sulfuric acid) on TLC, as white needles (1.80 mg). to stand for a few minutes which, after neutralization and concentration was treated by silica gel column chromatography (Waco Gel C-300, 50 g, solvent: chloroform-methanol system)